Induksi Umbi Mikro Tanaman Daun Dewa (Gynura pseudochina (Lour.) DC) Secara In Vitro dengan Perlakuan Sukrosa dan Daminozide

Donny Hartanto, Sandra Arifin Aziz, Diny Dinarti

Abstract


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The effects of sucrose and daminozide on in-vitro microtuber formation were evaluated for producing microtubers to supply year round microtubers and to facilitate sterilized explants exchange regionally and internationally. Uninodal stem segment explants were cultured on agar solidifi  ed Murashige and Skoog medium supplemented with 3% sucrose, 0.5 mg L-16-benzylaminopurine (BAP), 0.1 mg L-1 α-naphthaleneacetic acid (NAA) for bud multiplication for 3 months. Three nodals stem segment which already  formed  three  perfect leaves were cultured on agar solidifi  ed MS medium, 3% sucrose and 1 mg L-1 NAA for two weeks to induce root formation (until ± minimum 60% of the explants rooted). The next step was the induction of three nodal stem segments for microtuber formation on agar solidifi  ed MS medium supplemented with 5 mg L-1 BAP and two levels of sucrose, four concentrations of daminozide for 10 weeks. Sucrose at 6% resulted in the signifi  cantly highest number of microtuber. The daminozide 41.29 mg L-1 stimulated tuberization at base of the stems and reduce number of microtuber formation in stolon.

Keywords: daminozide, Gynura pseudochina (L.) DC, in vitro, microtuber, sucrose


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DOI: https://doi.org/10.24831/jai.v38i2.1799

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