Evaluation of Bacterial Biofilm as Biosensor for Detecting Phenol, Catechol, and 1,2-dihydroxynaphthalene

  • Dyah Iswantini Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB-University, Bogor, Indonesia
  • Ali Aulia Ghozali Graduated Program of Environmental and Natural Resources Management Sciences, IPB-University, Bogor, Indonesia
  • Cecep Kusmana Department of Silviculture, Faculty of Forestry, IPB-University, Bogor, Indonesia
  • Novik Nurhidayat Microbial Laboratory for Health, Central Research Department of Biology, Indonesian Institute of Sciences (LIPI), Cibinong, Indonesia

Abstract

Monitoring of water quality from the presence of polyaromatic hydrocarbon (PAHs) compounds and its derivates are important for keeping the healthy aquatic environment. Some of those derivates are phenol and several related compounds sharing simmilar structures. This reseach aimed for the detection of those phenol and several similar compounds monitoring due to PAHs degradation. Three identified bacterial isolates of Pseudomonas aeruginosa, Staphylococcus sciuri, and Bacillus amyloliquifaciens were selected based on their phenol degradation characters. On physiological properties all three isolates were observed to degrade several hydrophobic substances such as for naphthalene and anthracene. Yet, genetic analysis indicated that the phenolic degradating oxygenase gene was detected only in the P. aeruginosa and S. sciuri. Applying those isolates for biofilm as biosensor showed a sufficient analytical performance such as their limit of detection between 0.1-0.5 μM.

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Published
2021-09-22
How to Cite
Iswantini, D., Ghozali, A. A., Kusmana, C., & Nurhidayat, N. (2021). Evaluation of Bacterial Biofilm as Biosensor for Detecting Phenol, Catechol, and 1,2-dihydroxynaphthalene. HAYATI Journal of Biosciences, 28(4), 262-270. https://doi.org/10.4308/hjb.28.4.262-270