Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions

Eunike Marganingrum Andriani  Samodra; Dian Suroto; Tyas Utami; Rachma Wikandari; Endang Sutriswati Rahayu

doi:10.4308/hjb.30.1.65-70

Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions

Eunike Marganingrum Andriani Samodra Faculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1 Bulaksumur, Yogyakarta, Indonesia
Dian Suroto Faculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1 Bulaksumur, Yogyakarta, Indonesia. Center for Food and Nutrition Studies, Universitas Gadjah Mada, Teknika Utara Street , Yogyakarta, Indonesia. University Center of Excellence for Research and Application on Integrated Probiotic Industry, Universitas Gadjah Mada, Yogyakarta, Indonesia
Tyas Utami Faculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1 Bulaksumur, Yogyakarta, Indonesia. Center for Food and Nutrition Studies, Universitas Gadjah Mada, Teknika Utara Street , Yogyakarta, Indonesia. University Center of Excellence for Research and Application on Integrated Probiotic Industry, Universitas Gadjah Mada, Yogyakarta, Indonesia
Rachma Wikandari Faculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1 Bulaksumur, Yogyakarta, Indonesia. Center for Food and Nutrition Studies, Universitas Gadjah Mada, Teknika Utara Street , Yogyakarta, Indonesia
Endang Sutriswati Rahayu Faculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1 Bulaksumur, Yogyakarta, Indonesia. Center for Food and Nutrition Studies, Universitas Gadjah Mada, Teknika Utara Street , Yogyakarta, Indonesia. University Center of Excellence for Research and Application on Integrated Probiotic Industry, Universitas Gadjah Mada, Yogyakarta, Indonesia

DOI: https://doi.org/10.4308/hjb.30.1.65-70

Abstract

Probiotics are widely consumed in various food matrices to provide health benefits to the host. The viability of probiotic cells is influenced by several factors, including exposure to high temperatures during the production process and low temperatures during storage. In this study, we report the response to cold stress of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 after 24 h of storage at 4°C and -20℃. The cell number of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 in low-temperature condition had no significant differences than their initial number: 11.88 log CFU/ml and 11.62 log CFU/ml at 4°C; 11.51 log CFU/ml and 11.47 log CFU/ml at -20°C for Mut-3 and Mut-7 respectively. The results indicated the survival capacity of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 at low temperatures. The genes encoding cold shock proteins for the response to cold stress were evaluated by genome sequencing. The CspA/CspC genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 possibly play a role in maintaining cell resistance at low temperatures, since the genes products predicted to have conserved motifs in the RNA binding protein (RNP) -1 and RNP-2 responsible for cold response stress which are similar to those in other bacteria.

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Published

2022-08-19

How to Cite

SamodraE. M. A., SurotoD., UtamiT., WikandariR., & RahayuE. S. (2022). Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions. HAYATI Journal of Biosciences, 30(1), 65-70. https://doi.org/10.4308/hjb.30.1.65-70

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Vol. 30 No. 1 (2023): January 2023

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