Artefactual Suboptimal Fixation Effect to Nuclear Staining on Erythrocytes of Lutjanus kasmira (Forsskål 1775)

Huu-Quyet Do; Van-Thanh Vo; Nguyen Thi Le-Na; Hoang Thi Thuy-Duong

doi:10.4308/hjb.30.1.48-52

Artefactual Suboptimal Fixation Effect to Nuclear Staining on Erythrocytes of Lutjanus kasmira (Forsskål 1775)

Huu-Quyet Do Institute of Tropical Ecology, Vietnam-Russia Tropical Center, 63 Nguyen Van Huyen, Nghia Do Ward, Cau Giay District, Hanoi, Vietnam
Van-Thanh Vo Biology Faculty, Ho Chi Minh City University of Education, 280 An Duong Vuong, Ward 4, District 5, Ho Chi Minh, Vietnam
Nguyen Thi Le-Na Faculty of Biology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Vietnam
Hoang Thi Thuy-Duong Institute of Tropical Ecology, Vietnam-Russia Tropical Center, 63 Nguyen Van Huyen, Nghia Do Ward, Cau Giay District, Hanoi, Vietnam

DOI: https://doi.org/10.4308/hjb.30.1.48-52

Abstract

This research presents the unusual staining characteristic of the teleosts red blood cells–the common bluestripe snapper Lutjanus kasmira, caught at Toc Tan (Allison) Reef, Truong Sa Archipelago, Vietnam, due to side effects of the delayed staining procedure. In addition to the quantitative data measured, the results of microscopic image research showed that, on slides performed with the delayed Romanovsky staining procedure using Giemsa dye, the cell "nucleus" was not dye-stained as seen in the erythrocytes of much other fish. The AFM and SEM images of blood slides show that the structure of the nuclear zone and the nuclear membrane was destroyed. Although methanol is used as one of the common fixatives in biology, the delayed staining still causes artifactual side effects, disrupts the structure of the red blood cell nucleus and can cause confusion when studying cytogenetics.

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Published

2022-08-19

How to Cite

DoH.-Q., VoV.-T., Le-NaN. T., & Thuy-DuongH. T. (2022). Artefactual Suboptimal Fixation Effect to Nuclear Staining on Erythrocytes of Lutjanus kasmira (Forsskål 1775). HAYATI Journal of Biosciences, 30(1), 48-52. https://doi.org/10.4308/hjb.30.1.48-52

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Issue

Vol. 30 No. 1 (2023): January 2023

Section

Short Communication

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